Bradford法蛋白含量检测试剂盒

检测样本量： 500 Samples
主要检测设备及配套：可见分光光度计（1 mL玻璃比色皿）/酶标仪（96孔板）
预计测定时间： 6 h (100 Samples)
需自备试剂： 无
试剂储存条件： 按照标签要求储存

考马斯亮蓝G-250染料游离状态下呈棕红色，与蛋白质结合后变为蓝色，染料的最大吸收峰由465 nm变为595 nm，在一定浓度范围内蛋白质-染料复合物吸光值与蛋白质含量成正比，通过测定595 nm处吸光值的变化即可定量检测蛋白质的含量。

• 检测方法： Bradford
• 检测波长： 595 nm
• 信号响应： 递增型

标准物质： BSA
参考标准： y=3.9836x+0.8988 (R²=0.9997)
标准线性范围： 0.04-0.2 mg/mL
检测限： 0.02 mg/mL

① Bradford法测定蛋白浓度不受绝大部分样品中的化学物质的影响，样品中巯基乙醇的浓度可高达1 M，二硫苏糖醇的浓度可高达5 mM；但受略高浓度的去垢剂影响，需确保样品中SDS浓度低于0.1%，TritonX-100低于0.1%，Tween 20、60、80低于0.06%；

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