木质素中的酚羟基发生乙酰化后生成乙酰木质素，产物在280 nm处具有特征吸收峰，通过吸光值变化即可定量检测木质素的含量。本试剂盒方法中含有脱糖和脱脂步骤，能够有效排除干扰物质使结果更加准确可靠。①根据乙酰化程度不同，需调整试剂七加入量，控制A测定处于0.1-0.8之间可保证结果准确性：
·若A测定大于0.8：建议将反应液使用试剂七适当稀释后再进行吸光值测定，可以设置几个梯度进行预实验确定稀释倍数，使A测定小于0.8即可，计算时修改公式中稀释倍数D即可；例如吸取100 μL反应液加入900 μL试剂七后充分混匀再进行吸光值测定，该条件下稀释倍数为10；
·若A测定小于0.1：建议适当减少试剂七的加入量使A测定大于0.1，例如吸取20 μL上清液加入980 μL试剂七，改为吸取20 μL上清液加入480 μL试剂七进行预实验，计算时修改公式中VS7即可（带入实际试剂七使用体积）；
②乙酰化反应过程较为剧烈，建议使用带盖密封性较好的冻存管或玻璃试管作为反应容器，请确保无橡胶等易腐蚀材质（或不与橡胶材质接触），以免造成泄漏；
③称取样本时重量范围可参考4-10 mg范围内均可，将样本具体称量质量带入公式W即可；
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