植物蔗糖含量检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：可见分光光度计/1 mL玻璃比色皿(d=10 mm)
预计测定时间： 4 h (50 Samples)
需自备试剂： 无
试剂储存条件： 按照标签要求储存

蔗糖能够在酸性条件下水解为葡萄糖和果糖，果糖与间苯二酚反应生成有色物质，产物在480 nm处具有特征吸收峰，通过吸光值变化即可定量检测蔗糖的含量。

• 检测方法： 间苯二酚法
• 检测波长： 480 nm
• 信号响应： 递增型

标准物质： Sucrose
参考标准： y=0.4452x+0.0092 (R²=0.9993)
标准线性范围： 0.125-3.0 mg/mL
检测限： 0.015 mg/mL

①若测定吸光值超出标准吸光值线性范围：高于最高值建议将待测样本使用提取液适当稀释后再进行测定，低于最低值建议适当调整样本量及提取液添加比例后再进行测定，计算时相应修改；

[1] Gong X, Liu R, Han Y, et al. Examining starch metabolism in lotus roots (Nelumbo nucifera Gaertn.) during post-harvest storage at different temperatures[J]. Food Chemistry, 2024: 139494. (IF 8.8)

[2] Li L, Li Q, Chen B, et al. Identification of candidate genes that regulate the trade-off between seedling cold tolerance and fruit quality in melon (Cucumis melo L.)[J]. Horticulture Research, 2023: uhad093.(IF 8.7)

[3] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)

[4] Lv N, Zhang H, Zhou H, et al. Hot water mobilizes the metabolism of energy, soluble sugar, cell wall, and phenolics to cope with chilling injury in postharvest snap beans[J]. Journal of the Science of Food and Agriculture, 2024. (IF 3.3)

[5] Wang K, Lei C, Tan M, et al. Increased soluble sugar accumulation in postharvest peaches in response to different defense priming elicitors[J]. Horticulture, Environment, and Biotechnology, 2023, 64(1): 115-131.(IF 2.4)

[6] Shu B, Xie X, Dai J, et al. Host plant-induced changes in metabolism and osmotic regulation gene expression in Diaphorina citri adults[J]. Journal of Insect Physiology, 2024, 152: 104599.(IF 2.2)

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