总胆固醇含量检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：可见分光光度计/1 mL玻璃比色皿（d=10 mm）
预计测定时间： 3 h (50 Samples)
需自备试剂： 异丙醇（C₃H₈O，MW=60.06，CAS: 67-63-0）
试剂储存条件： 按照标签要求储存

胆固醇酯酶可催化胆固醇酯水解生成游离胆固醇（FC）和游离脂肪酸（FFA），胆固醇氧化酶可催化游离胆固醇氧化生成∆4-胆甾烯酮和H₂O₂，过氧化物酶催化H₂O₂氧化4-氨基安替比林和酚生成红色醌类化合物，产物在500 nm处具有特征吸收峰，通过吸光值变化即可定量检测总胆固醇的含量。

• 检测方法： 胆固醇氧化酶法
• 检测波长： 500 nm
• 信号响应： 递增型

标准物质： Cholesterol
参考标准： y=0.6602x+0.0211 (R²=0.9992)
标准线性范围： 0.0625-2.0 μmol/mL
检测限： 0.06 μmol/mL

①若∆A测定超出标准线性吸光值范围：高于最高值将待测样本使用提取液适当稀释后再进行测定，低于最低值建议适当增加样本量后再进行测定，计算时相应修改；

[1] Wang W, Wang Y, Su L, et al. Endothelial Cells Mediated by STING Regulate Oligodendrogenesis and Myelination During Brain Development[J]. Advanced Science, 2024: 2308508. (IF 14.3)

[2] Xu J, Khan H, Yang L. Hydrogel paper-based analytical devices: separation-free in situ assay of small-molecule targets in whole blood[J]. Analytical Chemistry, 2021, 93(44): 14755-14763.(IF 6.986)

[3] Yu J, Sun H, Yang Y, et al. Sesamolin alleviates nonalcoholic fatty liver disease through modulating gut microbiota and metabolites in high-fat and high-fructose diet-fed mice[J]. International Journal of Molecular Sciences, 2022, 23(22): 13853.(IF 6.208)

[4] Jiang J L, Zhou Y Y, Zhong W W, et al. Uridine diphosphate glucuronosyltransferase 1A1 prevents the progression of liver injury[J]. World Journal of Gastroenterology, 2024, 30(9): 1189. (IF 4.3)

[5] Song J, Qiu H, Du P, et al. Polyphenols extracted from Shanxi‐aged vinegar exert hypolipidemic effects on OA‐induced HepG2 cells via the PPARα‐LXRα‐ABCA1 pathway[J]. Journal of food biochemistry, 2022, 46(2): e14029.(IF 3.654)

[6] He H, Qi R, Cui J, et al. Lipid characteristics of lung tissue in silicosis rat model were studied based on lipid metabolomics[J]. Toxicology Letters, 2024, 391: 111-119.(IF 3.5)

[7] Wang X, Huangfu W, Zhao F. Correlation of ChREBP Gene Methylation with Pathological Characteristics of Type 2 Diabetes Mellitus[J]. Applied Biochemistry and Biotechnology, 2023: 1-12.(IF 3)

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