ATP含量检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：紫外分光光度计/1 mL 石英比色皿（d=10 mm）
预计测定时间： 5 h (50 Samples)
需自备试剂： 氯仿（CHCl₃，MW=119.38，CAS: 67-66-3）
试剂储存条件： 按照标签要求储存

己糖激酶（HK）能够催化葡萄糖和ATP生成6-磷酸葡萄糖，6-磷酸葡萄糖脱氢酶（G6PDH）进一步催化6-磷酸葡萄糖和NADP⁺生成6-磷酸葡萄糖酸和NADPH，NADPH在340 nm处具有特征吸收峰，通过吸光值变化即可定量检测ATP的含量。

• 检测方法： NADPH速率法
• 检测波长： 340 nm
• 信号响应： 递增型

标准物质： ATP
参考标准： y=0.4594x+0.0005 (R²=0.9999)
标准线性范围： 0.0625-2.0 µmol/mL
检测限： 0.003 µmol/mL

①试剂六配制后有效期较短，附赠一支作为备用，每支均可满足至少50个样本的测定；

②提取过程应严格在冰浴条件下进行；

③提取过程中第一次离心后的上清液若为浑浊属于正常现象，不影响检测结果；

④测定过程中试剂四应用液、试剂六应用液和待测样本应置于冰上放置，以免变性和失活；

⑤提取液出现晶体析出属于正常现象，50-60℃水浴加热至完全溶解，冷却至室温后使用即可；

⑥准确在10 s和190 s处完成吸光值测定，以确保实验结果的准确性和重复性；

⑦若A2测定超出标准吸光值线性范围：高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定，低于最低值建议适当增加样本量重新提取后再进行测定，计算时相应修改；

⑧提取液中含有蛋白沉淀组分，待测样本不能直接用于蛋白含量测定，若需测定蛋白含量需使用PBS或生理盐水单独提取后再进行测定；

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