BCA（Bicinchonininc Acid）蛋白含量检测法是用于总蛋白质定量的常用方法，BCA与二价铜离子混合即为BCA工作液，蛋白在碱性条件下能够将Cu2+还原为Cu+，Cu+与BCA试剂可形成紫色络合物，产物在562 nm处具有特征吸收峰，通过吸光值变化即可定量检测样品的蛋白浓度。
标准物质： BSA①Copper Solution与 PBS Diluent可置于2-8℃长期保存，若发现污染浑浊则应丢弃；BCA Solution在低温条件下出现结晶沉淀时，可37℃温育使其完全溶解，不影响使用；
②为了您的安全和健康，请穿实验服并戴一次性手套操作；
③BCA法测定蛋白含量不受绝大部分样品中化学物质的影响，样品中5% SDS、5% Triton X-100、5% Tween20、60、80等常用浓度去垢剂不影响检测结果，但EDTA和EGTA等螯合剂、DTT和巯基乙醇等还原剂和脂类会影响检测结果，需确保EDTA低于10 mM、无EGTA、二硫苏糖醇低于1 mM、巯基乙醇低于0.01%；若样品含有较多干扰物质且本身背景值较高，建议使用Bradford法蛋白含量检测试剂盒（Cat AKPR015）。
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