植物叶绿素含量检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：可见分光光度计/1 mL玻璃比色皿(d=10 mm)
预计测定时间： 5 h (50 Samples)
需自备试剂： 无水乙醇（C₂H₆O，MW=46.07，CAS: 64-17-5）；
丙酮（C₃H₆O，MW=58.08，CAS: 67-64-1）
试剂储存条件： 按照标签要求储存

叶绿素a和叶绿素b分别在645 nm和663 nm处具有特征吸收峰，根据经验公式即可计算叶绿素a和叶绿素b以及总叶绿素的含量。

• 检测方法： 乙醇提取法
• 检测波长： 645 nm & 663 nm
• 信号响应： 递增型

①叶绿素对光敏感，研磨和提取等操作尽量避光或者在弱光下进行；

②提取时应浸提至组织残渣完全变白，否则会因提取不充分而影响测定结果；

③提取液冲洗研钵时应冲洗至所有的绿色物质转移至试管/离心管中；

④若测定吸光值超过1.0，建议将浸提液使用提取液适当稀释后再进行测定，计算时相应调整（按实际稀释倍数调整计算公式中D）；若测定吸光值小于0.05时，可适当减少提取过程中提取液的用量后再进行测定，计算时相应调整（按实际提取后总体积调整计算公式中V提）；

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