类黄酮与铝离子能够在碱性亚硝酸盐溶液中形成红色络合物，产物在510 nm处具有特征吸收峰，通过吸光值变化即可定量检测植物类黄酮的含量。
标准物质： Rutin①若A测定或∆A测定超出标准吸光值线性范围：高于最高值建议将待测样本使用提取液适当稀释后再进行测定；低于最低值建议适当增加样本量重新提取后再进行测定，计算时相应修改；
②提取液中含有蛋白沉淀组分，样本蛋白浓度测定需使用PBS单独提取后再进行测定；
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