土壤碱性磷酸酶活性检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：可见分光光度计/1 mL玻璃比色皿(d=10 mm)
预计测定时间： 25 h (50 Samples)
需自备试剂： 无水乙醇（C₂H₆O，MW=46.07，CAS: 64-17-5）；
甲苯（C₇H₈，MW=96.14，CAS:108-88-3）
试剂储存条件： 按照标签要求储存

土壤碱性磷酸酶（S-AKP/ALP）能够在碱性环境中催化磷酸苯二钠水解生成苯酚和磷酸氢二钠，通过测定酚的生成量即可表征土壤碱性磷酸酶的活性。

• 检测方法： 磷酸苯二钠法
• 检测波长： 660 nm
• 信号响应： 递增型

标准物质： Phenol
参考标准： y=0.7648x+0.0215（R²=0.9992）
标准线性范围： 0.1-1.0 μmol/mL
检测限： To Be Updated

①若测定吸光值超出标准吸光值线性范围：高于最高值建议将待测样本适当稀释后再进行测定，低于最低值建议适当增加样本后再进行测定，计算时相应修改即可；

[1] Ma X, Song Y, Shen Y, et al. Fine-scale measurements unravel the side effects of biochar capping on the bioavailability and mobility of phosphorus in sediments[J]. Biochar, 2024, 6(1): 49. (IF 12.7)

[2] Li X, Fan S, Zhang Y, et al. Performance and microbial metabolic mechanism of imidacloprid removal in a microbial electrolysis cell-integrated adsorption biological coupling system[J]. Bioresource Technology, 2023, 386: 129513.(IF 11.4)

[3] Huang B, Chen Y, Pei Z, et al. Application of microbial organic fertilizers promotes the utilization of nutrients and restoration of microbial community structure and function in rhizosphere soils after dazomet fumigation[J]. Frontiers in Microbiology, 2023, 13: 1122611.(IF 5.2)

[4] Yu S U N, Li J I, CHANG J, et al. Compositional and functional succession of soil bacterial communities during long-term rice cultivation on saline-alkali soils: Insights derived from a new perspective of the core bacterial taxa[J]. Pedosphere, 2024. (IF 5.2)

[5] Zhou J, Miao Y, Guo L, et al. Evaluating the Enzyme Activities and Soil Physicochemical Properties of Four Typical Halophytic Communities in Saline-Sodic Soil[J]. Agronomy, 2024, 14(1): 141.(IF 3.7)

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