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淀粉含量检测试剂盒

Starch Content Assay Kit
货号:AKSU015C 检测设备:可见分光光度计 可检测样本数:50 Samples
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产品信息
检测样本量: 50 Samples
主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
预计测定时间: 4 h (50 Samples)
需自备试剂: 浓硫酸(H2SO4,MW = 98.08,CAS: 7664-93-9)
试剂储存条件: 按照标签要求储存
检测原理
使用洗脱液将样品中可溶性糖与淀粉分开,淀粉经酸水解为葡萄糖,进一步与蒽酮反应生成蓝绿色糠醛衍生物,产物在620 nm处具有特征吸收峰,通过吸光值变化即可定量检测淀粉的含量。
  • 检测方法: 蒽酮比色法
  • 检测波长: 620 nm
  • 信号响应: 递增型
标准曲线
标准物质: Glucose
参考标准: y=8.3736x+0.0073 (R2=0.9998)
标准线性范围: 0.01-0.12 mg/mL
检测限: 0.003 mg/mL
注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
注意事项

①若A测定或∆A测定超出标准线性吸光值范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;

②浓硫酸具有强腐蚀性,请做好防护措施并谨慎操作;

③待测样本稀释倍数可参照公式计算

最大稀释倍数(DMax)=74"×W×A"

最小稀释倍数(DMin)=6"×W×A"

注释:W:样品质量,g;A:预估淀粉含量,mg/g;例:预估淀粉含量为400 mg/g(40%),称取0.05 g样本进行淀粉提取,DMin=120,DMax=1480,即待测样本稀释倍数可选择120-1400倍。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
引用文献

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