β-半乳糖苷酶活性检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：酶标仪/96孔板
预计测定时间： 5 h (50 Samples)
需自备试剂： 无
试剂储存条件： 按照标签要求储存

β-半乳糖苷酶能够分解对-硝基苯-β-D-吡喃半乳糖苷生成对-硝基苯酚，产物在400 nm处具有特征吸收峰，通过吸光值变化即可表征β-半乳糖苷酶的活性。

• 检测方法： 对-硝基苯酚法
• 检测波长： 400 nm
• 信号响应： 递增型

标准物质： P-Nitrophenol
参考标准： y=0.003x-0.0003 (R²=0.9999)
标准线性范围： 12.5-300 nmol/mL
检测限： To Be Updated

①若测定吸光值超出标准线性吸光值范围，建议适当增加样本量或将粗酶液适当稀释后再进行测定，计算时相应修改；

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