抗坏血酸过氧化物酶可催化H2O2氧化抗坏血酸生成单脱氢抗坏血酸，通过测定抗坏血酸的氧化速率即可表征抗坏血酸过氧化物酶的活性。①若ΔA大于1.2，建议将粗酶液使用试剂一适当稀释后再进行测定；若ΔA小于0.05，建议适当延长酶促反应时间（准确反应120 s，可以延长至5 min以上）或制备更高浓度的样本后再进行测定，计算时相应修改；
②准确在规定时间点完成吸光值测定，以确保检测结果的准确性和重复性；
③粗酶液应置于冰上待测，建议提取完成后当天完成活性检测；
④试剂二配制后有效期短，为便于试验安排，附赠一瓶试剂二作为备用，每瓶均可完成至少50个样本的检测；
⑤若吸光值超过3，建议检查比色皿是否为石英比色皿（Q），玻璃比色皿（G）会因材质问题造成吸光值超出设备量程；
[1] Chen L, Yue M, Liu X, et al. Dynamic distribution and temporal transcriptome adaptations elucidate lithium accumulation pattern in Cardamine violifolia[J]. Environmental Chemistry and Ecotoxicology, 2025. (IF 9.0)
[2] Zhou D, Hu Y, Zhou T, et al. Deep eutectic solvent extraction of polysaccharides from Gastrodiae elata with ultrasonic and enzymes assistance: Process optimization, structure, and antioxidant activity[J]. Ultrasonics Sonochemistry, 2025: 107383. (IF 8.7)
[3] Liang X, Li Y, Wang L, et al. Knockout of stigmatic ascorbate peroxidase 1 (APX1) delays pollen rehydration and germination by mediating ROS homeostasis in Brassica napus L[J]. The Plant Journal, 2024. (IF 7.2)
[4] Yao X, Li R, Liu Y, et al. Feedback regulation of the isoprenoid pathway by SsdTPS overexpression has the potential to enhance plant tolerance to drought stress[J]. Physiologia Plantarum, 2024, 176(2): e14277. (IF 6.4)
[5] Li C, Li J, Du X, et al. Chloroplast thylakoidal ascorbate peroxidase, PtotAPX, has enhanced resistance to oxidative stress in Populus tomentosa[J]. International Journal of Molecular Sciences, 2022, 23(6): 3340.(IF 6.208)
[6] Yao X, Zhou M, Ruan J, et al. Physiological and Biochemical Regulation Mechanism of Exogenous Hydrogen Peroxide in Alleviating NaCl Stress Toxicity in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)[J]. International Journal of Molecular Sciences, 2022, 23(18): 10698.(IF 6.208)
[7] Huang Y, Hu B, Li T, et al. Intercropping and Nano Zinc Oxide Application Enhance Plant Resistance and Alleviate Pesticide Stress by Altering the Soil Microenvironment[J]. Journal of Agricultural and Food Chemistry, 2025. (IF 6.2)
[8] Shu P, Li Y, Sheng J, et al. Tomato SlMAPK3 Modulates Cold Resistance by Regulating the Synthesis of Raffinose and the Expression of SlWRKY46[J]. Journal of Agricultural and Food Chemistry, 2024. (IF 6.1)
[9] Li J, Cai B, Chang S, et al. Mechanisms associated with the synergistic induction of resistance to tobacco black shank in tobacco by arbuscular mycorrhizal fungi and β-aminobutyric acid[J]. Frontiers in Plant Science, 2023, 14.(IF 5.6)
[10] Shen L, Zhou Y, Yang X. Genome-wide identification of ascorbate peroxidase (APX) gene family and the function of SmAPX2 under high temperature stress in eggplant[J]. Scientia Horticulturae, 2024, 326: 112744.(IF 4.3)
[11] Xu J, Kang Z, Zhu K, et al. RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity–alkalinity stress[J]. Plant Physiology and Biochemistry, 2021, 164: 237-246.(IF 4.27)
[12] Xiao H, Deng W, Ahmad B, et al. Cucurbita ficifolia Rootstock Enhances Resistance to Low-Temperature Stress in Cucumber[J]. Horticulturae, 2025, 11(3): 242. (IF 3.1)