甘油三酯含量检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：可见分光光度计/1 mL玻璃比色皿（d=10 mm）
预计测定时间： 3 h (50 Samples)
需自备试剂： 正庚烷（C₇H₁₆，MW=100.2，CAS: 142-82-5）；
异丙醇（C₃H₈O，MW=60.01，CAS: 67-63-0）
试剂储存条件： 按照标签要求储存

甘油三酯经KOH皂化水解生成甘油及脂肪酸，过碘酸氧化甘油生成甲醛，在铵离子存在下甲醛能够与乙酰丙酮缩合生成黄色物质，产物在420 nm处具有特征吸收峰，通过吸光值的变化即可定量检测甘油三酯的含量。

• 检测方法： 乙酰丙酮法
• 检测波长： 420 nm
• 信号响应： 递增型

标准物质： Triglyceride
参考标准： y=0.8603x+0.0253 (R²=0.9992)
标准线性范围： 0.0625-1.5 mg/mL
检测限： 0.06 mg/mL

①为确保试验结果重复性，加入试剂二后需剧烈震荡，振荡幅度、时间、反复次数以及等待分层时间均应保持一致，检测体系中65℃水浴后冷却时间应保持一致；

②若测定吸光值超出标准吸光值线性范围：高于最高值建议将TG待测液使用试剂一适当稀释后再进行测定，低于最低值建议适当增加样本量后再进行测定，计算时相应修改；

③检测过程中使用具有易挥发性物质，建议在通风橱中进行测定，并做好防护措施；

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