乙酰辅酶A含量检测试剂盒

检测样本量： 24 Samples
主要检测设备及配套：紫外分光光度计/1 mL 石英比色皿（d=10 mm）
预计测定时间： 2 h (25 Samples)
需自备试剂： 无
试剂储存条件： 按照标签要求储存

苹果酸脱氢酶能够催化苹果酸和NAD生成草酰乙酸和NADH，柠檬酸合酶可催化乙酰辅酶A和草酰乙酸生成柠檬酸和辅酶A，通过苹果酸脱氢酶和柠檬酸合酶的偶联反应体系，乙酰辅酶A含量和NADH的生成速率成正比，NADH在340 nm处具有特征吸收峰，通过吸光值的变化速率即可定量检测乙酰辅酶A的含量。

• 检测方法： NADH速率法
• 检测波长： 340 nm
• 信号响应： 递增型

①准确在20 s和80 s处完成吸光值的测定，以确保实验结果的准确性和重复性；

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