多酚氧化酶能够催化邻苯二酚生成醌，产物在410 nm处具有特征吸收峰，通过吸光值变化即可表征多酚氧化酶的活性。①不同样本的多酚氧化酶最佳反应温度略有差别，反应温度可在25-37℃之间进行调整；
②若A测定大于1.5或ΔA大于1.0，建议将粗酶液适当稀释后再进行测定；若ΔA小于0.02，建议适当延长酶促反应时间或适当增加样本量后再进行测定，计算时相应修改；
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