超氧阴离子能够氧化盐酸羟胺生成NO2-，NO2-在对氨基苯磺酸和α-萘胺的作用下，生成红色偶氮化合物，产物在530 nm处具有特征吸收峰，根据吸光值变化即可定量检测O2-的含量。
标准物质： Sodium Nitrite①若测定吸光值超出标准吸光值线性范围：高于最高值建议将待测样本使用提取液适当稀释后再进行测定，低于最低值建议适当增加样本量后再进行测定，计算时相应修改；
②样本提取后应立即测定，请勿将待测样本长时间低温保存，以免影响测定结果；
③氯仿具有一定毒性，建议在通风橱中进行操作，并做好相应防护措施；
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