总巯基含量检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：可见分光光度计/1 mL玻璃比色皿（d=10 mm）
预计测定时间： 3 h (50 Samples)
需自备试剂： 无
试剂储存条件： 按照标签要求储存

巯基基团能够与5,5'-二硫代-双-（2-硝基苯甲酸）（5,5'-Dithiobis-2-Nitrobenoic Acid，DTNB）反应生成黄色的2-硝基-5-巯基苯甲酸，产物在412 nm处具有特征吸收峰，通过吸光值变化即可定量检测总巯基的含量。

• 检测方法： DTNB法
• 检测波长： 412 nm
• 信号响应： 递增型

标准物质： Reduced Glutathione
参考标准： y=2.8786x+0.2456 (R²=0.9999)
标准线性范围： 0.025-0.4 µmol/mL
检测限： 0.005 μmol/mL

①若测定吸光值超出标准吸光值线性范围：高于最高值建议将待测样本使用提取液适当稀释后再进行测定；低于最低值建议制备更高浓度的样本后再进行测定，计算时相应修改；

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