漆酶能够催化ABTS生成ABTS自由基，产物在420 nm处具有特征吸收峰，通过吸光值变化速率即可表征漆酶的活性。①若A2测定大于1.0，建议将粗酶液使用提取液适当稀释后再进行测定，计算时相应修改；
②空白管为检测各试剂组分质量的检测孔，正常情况下，OD值变化不超过0.05；
③准确在10 s和190 s完成吸光值测定，以确保实验结果的准确性和重复性；
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