ABTS在氧化剂作用下被氧化为稳定的蓝绿色阳离子ABTS自由基，在405 nm或734 nm处具有特征吸收峰，抗氧化物存在时会抑制ABTS自由基的生成使反应体系褪色，405 nm处吸光值随之下降，在一定范围内吸光值的变化与自由基被清除的程度成正比，通过吸光值的下降程度即可表征样本的ABTS自由基清除能力，并提供Trolox作为阳性对照量化抗氧化物质的清除能力。
标准物质： Trolox①样品提取过程建议在冰上完成操作，且提取后应当天完成测定；
②若待测样本ABTS自由基清除率（DS%）大于90%，建议将待测样本使用提取液稀释后再进行测定；若待测样本ABTS自由基清除率（DS%）小于5%，建议适当增加烘干样本质量或液体样本体积重新提取后再进行测定，计算时相应修改；
③试剂四应用液和试剂五应用液配制时，建议最少取10 μL进行配制；
④不同样本ABTS自由基清除能力可能相差较大，若需要比较不同样本的ABTS自由基清除能力，建议对于同一批植物样本加入等量的样本，液体样本加入相同体积，提取物或者药物配制为相同浓度；将样本根据预实验结果进行适当调整，比较同样浓度（相同稀释倍数）的清除率大小；
⑤试剂三配制后有效期短，为便于试验安排，附赠2支试剂三作为备用；
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