谷胱甘肽和5,5’-二硫代-双-（2-硝基苯甲酸）（5,5’-dithiobis-2-nitrobenoic acid，DTNB）反应生成黄色的2-硝基-5-巯基苯甲酸和谷胱甘肽二硫化物（GSSG），2-硝基-5-巯基苯甲酸在412 nm处具有特征吸收峰，通过吸光值变化即可定量检测还原型谷胱甘肽的含量。
标准物质： Reduced Glutathione①样品处理过程应在冰上迅速完成，提取后待测样本应置于冰上保存且建议当天完成测定；
②试剂一中含有蛋白质沉淀剂，待测样本不能用于蛋白浓度测定，需另取组织使用1×PBS或生理盐水按照与待测样本相同的样本浓度重新提取后，再进行蛋白含量测定；
③若测定吸光值超出标准吸光值线性范围：高于最高值建议将待测样本使用试剂一适当稀释后再进行测定；低于最低值建议制备更高浓度样本后再进行测定，计算时相应修改；
[1] Wang Y, Lu C, Guo S, et al. Leafhopper salivary vitellogenin mediates virus transmission to plant phloem[J]. Nature Communications, 2024, 15(1): 3.(IF 16.6)
[2] Wang Y, Liu X, Li K, et al. Self-Sulfhydrated, Nitro-Fixed Albumin Nanoparticles as a Potent Therapeutic Agent for the Treatment of Acute Liver Injury[J]. ACS nano, 2024. (IF 15.8)
[3] Wang Y, Qian D, Wang X, et al. Biomimetic Trypsin-Responsive Structure-Bridged Mesoporous Organosilica Nanomedicine for Precise Treatment of Acute Pancreatitis[J]. ACS nano, 2024. (IF 15.8)
[4] Wang X, Wu H, Yu Z, et al. Plant viruses exploit insect salivary GAPDH to modulate plant defenses[J]. Nature Communications, 2024, 15(1): 6918. (IF 14.7)
[5] Wang Y, Ma X, Wang X, et al. Traditional Chinese Medicine Borneol‐Based Polymeric Micelles Intracerebral Drug Delivery System for Precisely Pathogenesis‐Adaptive Treatment of Ischemic Stroke[J]. Advanced Science, 2025: 2410889. (IF 14.3)
[6] Shi L, Zhao X, Zhong K, et al. Physiological mechanism of the response to Cr (VI) in the aerobic denitrifying ectomycorrhizal fungus Pisolithus sp. 1[J]. Journal of Hazardous Materials, 2022, 429: 128318.(IF 14.224)
[7] Sun W, Wu G, Xu H, et al. Malate-mediated CqMADS68 enhances aluminum tolerance in quinoa seedlings through interaction with CqSTOP6, CqALMT6 and CqWRKY88[J]. Journal of Hazardous Materials, 2022, 439: 129630.(IF 14.224)
[8] Chen K, Zheng C, Lv Y, et al. Complement opsonized protein corona activated by precoated immunoglobulin enables neutrophil-hitchhiking for rapid and enhanced drug delivery for acute liver failure recovery[J]. Nano Today, 2024, 59: 102512. (IF 13.2)
[9] Wang S, Ding Y, Xu L, et al. A novel sunflower-like nanocarrier based on dual milk-derived proteins for improved bio-accessibility, stability and antioxidant activity of anthocyanin[J]. Food Hydrocolloids, 2024, 155: 110192. (IF 10.7)
[10] Cheng J, Chen S, Geng M, et al. Carrier‐Free Self‐Assembled Nanoparticles for Triple‐Amplified Tumor Chemodynamic Therapy and Cuproptosis Induction[J]. Advanced Healthcare Materials, 2025: 2501507. (IF 10.0)
[11] Meng Y, Huang J, Ding J, et al. Poly-thymine DNA templated MnO2 biomineralization as a high-affinity anchoring enabling tumor targeting delivery[J]. Journal of Colloid and Interface Science, 2023, 637: 441-452.(IF 9.9)
[12] Zhang Y, Tian X, Huang P, et al. Biochemical and transcriptomic responses of buckwheat to polyethylene microplastics[J]. Science of The Total Environment, 2023, 899: 165587.(IF 9.8)
[13] Ji C, Miao J, Xia B, et al. Evaluation of the toxic effects of fluindapyr, a novel SDHI fungicide, to the earthworms Eisenia fetida[J]. Science of The Total Environment, 2023, 899: 165697.(IF 9.8)
[14] Sun J, Yan B, Chen H, et al. Insight into the mechanisms of combined toxicity of cadmium and flotation agents in luminescent bacteria: Role of micro/nano particles[J]. Science of The Total Environment, 2024: 173588. (IF 9.8)
[15] Chen L, Yue M, Liu X, et al. Dynamic distribution and temporal transcriptome adaptations elucidate lithium accumulation pattern in Cardamine violifolia[J]. Environmental Chemistry and Ecotoxicology, 2025. (IF 9.0)
[16] Hu L, Yang K, Chen Y, et al. K+-H+ coupling strategy for immune regulation and bone defect repair[J]. Materials Today Bio, 2025, 32: 101744. (IF 8.7)
[17] Zhu L, Long P, Hu M, et al. Insight into selenium biofortification and the selenite metabolic mechanism of Monascus ruber M7[J]. Food Chemistry, 2024, 455: 139740. (IF 8.5)
[18] Wang Y, Wei Y, Wu Y, et al. Multifunctional Nano-Realgar Hydrogel for Enhanced Glioblastoma Synergistic Chemotherapy and Radiotherapy: A New Paradigm of an Old Drug[J]. International Journal of Nanomedicine, 2023: 743-763.(IF 8)
[19] Peng L, Xiao H, Xu Y, et al. The pentatricopeptide repeat protein PPR767 modulates plant architecture and drought resistance in rice[J]. Plant Physiology, 2025: kiaf187. (IF 6.5)
[20] Zhong Y, Xu J, Zhao X, et al. Balancing Damage via Non-Photochemical Quenching, Phenolic Compounds and Photorespiration in Ulva prolifera Induced by Low-Dose and Short-Term UV-B Radiation[J]. International Journal of Molecular Sciences, 2022, 23(5): 2693.(IF 6.208)
[21] Chen X L, Wu L J, Miao L L, et al. Chronic polystyrene microplastics exposure-induced changes in thick-shell mussel (Mytilus coruscus) metaorganism: A holistic perspective[J]. Ecotoxicology and Environmental Safety, 2024, 284: 116961. (IF 6.2)
[22] Yue Z, Guo Y, Zhang Y, et al. Endophytic Pantoea sp. EEL5 isolated from Elytrigia elongata improves wheat resistance to combined salinity-cadmium stress by affecting host gene expression and altering the rhizosphere microenvironment[J]. Industrial Crops and Products, 2025, 234: 121585. (IF 6.2)
[23] Liu Y, Li T, Zhu H, et al. Cysteine facilitates the lignocellulolytic response of Trichoderma guizhouense NJAU4742 by indirectly up-regulating membrane sugar transporters[J]. Biotechnology for Biofuels and Bioproducts, 2023, 16(1): 159.(IF 6.1)
[24] Luo Y, Lei Y, Zhou H, et al. ARRB1 downregulates acetaminophen-induced hepatoxicity through binding to p-eIF2α to inhibit ER stress signaling[J]. Cell Biology and Toxicology, 2024, 40(1): 1.(IF 6.1)
[25] Li C, Cui Z, Deng S, et al. Programmed Cell Death Protein 2-like Promotes Inflammation and Oxidative Stress in Vascular Endothelial Cells[J]. ACS Pharmacology & Translational Science, 2023, 6(10): 1453-1470.(IF 6)
[26] Zhu Y, Dong C, Xu Z, et al. Human Umbilical Cord Mesenchymal Stem Cells Alleviate Diabetic Nephropathy by Inhibiting Ferroptosis via the JNK/KEAP1/NRF2 Signaling Pathway[J]. Antioxidants & Redox Signaling, 2024. (IF 5.9)
[27] Liu Y, Xia S, Xiao M, et al. Synthesis of a metal–organic framework Cu-Mi-UiO-66-based fluorescent nanoprobe for the simultaneous sensing and intracellular imaging of GSH and ATP[J]. Nanoscale, 2024.(IF 5.8)
[28] Pan T, Li M, Guo J, et al. Bacillus safensis from Sauerkraut Alleviates Acute Lung Injury Induced by Methicillin-Resistant Staphylococcus aureus through the Regulation of M2 Macrophage Polarization via Its Metabolite Esculin[J]. Journal of Agricultural and Food Chemistry, 2024. (IF 5.7)
[29] Wang H, Wang Z, Gao Y, et al. STZ-induced diabetes exacerbates neurons ferroptosis after ischemic stroke by upregulating LCN2 in neutrophils[J]. Experimental Neurology, 2024: 114797. (IF 5.3)
[30] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)
[31] Yue Z, Chen C, Liu Y, et al. Phosphorus solubilizing Bacillus altitudinis WR10 alleviates wheat phosphorus deficiency via remodeling root system architecture, enhancing phosphorus availability, and activating the ASA-GSH cycle[J]. Plant and Soil, 2023: 1-13.(IF 4.9)
[32] Tao D, Wang Y, Liu J, et al. Mechanism of CuSO4 cytotoxicity in goat erythrocytes after high-level in vitro exposure to isotonic media[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111730.(IF 4.872)
[33] Shao M, Qi K, Wang L, et al. E3 ubiquitin ligase CHIP interacts with transferrin receptor 1 for degradation and promotes cell proliferation through inhibiting ferroptosis in hepatocellular carcinoma[J]. Cellular Signalling, 2024: 111148. (IF 4.8)
[34] Qi S, Li D, Deng F, et al. Brusatol modulates the Nrf2/GCLC pathway to enhance ferroptosis in the treatment of oral squamous cell carcinoma[J]. European Journal of Pharmacology, 2025: 177935. (IF 4.7)
[35] Zhang Q, Lin J, Yan J, et al. Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma[J]. Microorganisms, 2024, 12(3): 518. (IF 4.5)
[36] Qin X, Hou R, Qu Z, et al. Structure-based molecular hybridization design and synthesis of Keap1-Nrf2 inhibitors for anti-inflammatory treatment[J]. Bioorganic Chemistry, 2025: 108350. (IF 4.5)
[37] He Y H, Ou L L, Jiang J L, et al. Bletilla striata polysaccharides alleviate metabolic dysfunction-associated steatotic liver disease through enhancing hepatocyte RelA/HNF1α signaling[J]. World Journal of Gastroenterology, 2025, 31(4): 93179. (IF 4.3)
[38] Shi J, Zhou Y, Yang S, et al. The key metabolic pathway of roots and leaves responses in Arachis hypogaea under Al toxicity stress[J]. BMC Plant Biology, 2025, 25(1): 439. (IF 4.3)
[39] You X X, Li X G, Zhang X K, et al. Arsenic Stress Resistance in the Endophytic Fungus Cladosporium cladosporioides: Physiological and Transcriptomic Insights into Heavy Metal Detoxification[J]. Journal of Fungi, 2025, 11(5): 374. (IF 4.2)
[40] Wu S, Liu H, Yi J, et al. β-arrestin1 protects intestinal tight junction through promoting mitofusin 2 transcription to drive parkin-dependent mitophagy in colitis[J]. Gastroenterology Report, 2024, 12: goae084. (IF 3.8)
[41] Shi L, Wang X, Dai Y, et al. Comparison of the aquatic toxicity of diquat and its metabolites to zebrafish Danio rerio[J]. Scientific Reports, 2024, 14(1): 31358. (IF 3.8)
[42] Zhang W, Yu P, Liu W, et al. Mechanism of sodium nitroprusside regulating ginseng quality[J]. Scientific Reports, 2025, 15(1): 1562. (IF 3.8)
[43] Li L, Wu S, Wang S, et al. Molecular Mechanism of Exogenous Selenium Affecting the Nutritional Quality, Species and Content of Organic Selenium in Mustard[J]. Agronomy, 2023, 13(5): 1425.(IF 3.7)
[44] Zhou H, Wan S, Luo Y, et al. Hepatitis B virus X protein induces ALDH2 ubiquitin-dependent degradation to enhance alcoholic steatohepatitis[J]. Gastroenterology Report, 2023, 11: goad006.(IF 3.6)
[45] Cheng H, Shi X, Li L. The Effect of Exogenous Selenium Supplementation on the Nutritional Value and Shelf Life of Lettuce[J]. Agronomy, 2024, 14(7): 1380. (IF 3.3)
[46] Cheng H, Chang S, Shi X, et al. Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions[J]. Horticulturae, 2024, 10(3): 209. (IF 3.1)
[47] Zhang W, Liu W, Wang L, et al. Effects of water stress on secondary metabolism of Panax ginseng fresh roots[J]. PloS one, 2024, 19(11): e0312023. (IF 2.9)
[48] Yang Z, Sun G, Tao J, et al. Dietary Tea Polyphenols Improve Growth Performance and Intestinal Microbiota Under Chronic Crowding Stress in Hybrid Crucian Carp[J]. Animals, 2025, 15(13): 1983. (IF 2.7)
[49] Zhang J, Mo J, Liu Y, et al. Inhibition of microRNA-139-5p by glucagon-like peptide-1 ameliorates oxidative stress-induced vascular endothelial cell damage via targeting SOD1/GCLc[J]. Endocrine Connections, 2025, 14(5). (IF 2.6)
[50] Cheng Z, Zhang X, Chen P, et al. Sarcoma protein kinase inhibition alleviates liver fibrosis by promoting hepatic stellate cells ferroptosis[J]. Open Life Sciences, 2023, 18(1): 20220781.(IF 2.2)
[51] Zheng H, Xu Y, Fan S, et al. Potential protective role of chlorogenic acid against cyclophosphamide-induced reproductive damage in male mice[J]. Toxicology Research, 2024, 13(5): tfae176. (IF 2.2)