NAD-苹果酸脱氢酶活性检测试剂盒

检测样本量： 50 Samples
主要检测设备及配套：紫外分光光度计/1 mL 石英比色皿（d=10 mm）
预计测定时间： 4 h (50 Samples)
需自备试剂： 无
试剂储存条件： 按照标签要求储存

NAD-苹果酸脱氢酶能够催化NADH还原草酰乙酸生成苹果酸，NADH在340 nm处具有特征吸收峰，通过吸光值的变化即可表征NAD-苹果酸脱氢酶的活性。

• 检测方法： NADH速率法
• 检测波长： 340 nm
• 信号响应： 递减型

①粗酶液制备过程必须在冰上进行，保持低温环境以防止酶变性失活；

②试验过程中试剂二、试剂三和粗酶液需置于冰上放置，以防止变性和失活；

③若ΔA大于0.5，建议将粗酶液适当稀释后再进行测定，计算时相应修改；

④准确在10 s和70 s处完成吸光值测定，以确保实验结果的准确性和重复性；

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